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Handheld Fluorometer for Phycocyanin

$1,095.00 per unit plus S&H

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Fluorometer

Beagle’s fluorometer is the most cost-effective instrument on the market.  It functions similarly to more expensive units, has greater range and is calibrated with a phycocyanin calibrator that Beagle supplies.  It is easy to use and ideal for labs, classrooms, or HAB monitoring programs where good science is required but budgets are limited.

Handheld Fluorometer w/ Phycocyanin CalibratorPurchase Includes:

  • Carrying Case
  • Transfer Pipet
  • 0.2 mL tubes
  • Power Adaptor / USB
  • User Manual
  • This flurormoter is an integral component of a broader HAB strategy!

To place an order please call Beagle at (614) 682-6588 or send us a purchase order to info@beaglebioproducts.com.

Microcystin BloomApplications

  • Drinking & Recreational Water Monitoring
  • Environmental Studies & Education
  • Photosynthesis Research & Education
  • Monitoring Agriculture Systems
  • Plant & Algae Cultivation
  • Ballast Water Monitoring (freshwater only)

Application Notes

Specifications

  • Product Type: Single Tube Fluorometer
  • Sample Volume: 0.2 mL tube
  • Excitation Wavelength(nm): 600
  • Detection Wavelength(nm): 650
  • Dynamic Range: 10 to 100,000 ppb
  • Read Out: RFU or Direct Concentration
  • Calibration: Two-Point Calibration (Zero plus Beagle’s Phycocyanin Calibrator)
  • User Interface: Touch Screen LCD Display
  • Power: 4 AA Batteries or 5V DC Power Adaptor
  • Computer Interface: USB Interface to retrieve up to 3 assays.
  • Dimensions (L x W x H): 185mm x 90mm x 35mm

Background

Phycocyanin absorbs strongly at 620nm

Phycocyanin absorbs strongly at 620nm

Fluorescence can be described in two parts: absorption and emission.  For something to fluoresce it first must absorb light.  The light that we can see is just a narrow part of the entire electromagnetic (light) spectrum that ranges from radio and microwaves through infrared, visible, and ultraviolet light to x-rays and gamma rays.  Light is classified into these groups by wavelengths that range in size from 100s of meters to a trillion times smaller.  Visible light occurs from about 400 nm for violet light to about 750 nm for red with each color in between having its own range of wavelengths.  When a compound absorbs visible light, it appears colored.  Interestingly, the color it appears is complementary to the color it absorbs.  As an example, phycocyanin is a protein that occurs in cyanobacteria that is involved in absorbing light for photosynthesis.  It absorbs orange light most strongly at 620 nm. The complementary color of this orange color is blue, so phycocyanin appears blue.

The complementary color of 620 nm is blue

The complementary color of 620 nm is blue

The second part of fluorescence is the ability to emit light.  Once light energy is absorbed, it has to be dissipated, which usually occurs as heat.  But some compounds dissipate the energy by converting it back to light.  This light is then emitted from the compound making it appear as if it has a slight glow.  Many types of fluorescence material are encountered everyday:  powder in fluorescent lights, dyes in highlighter ink, security features in official documents, compounds in minerals/gemstones, and phycocyanin in algae blooms.

Not everything can fluoresce, and those that can do not absorb and emit the same type of light.  For example chlorophyll, another pigment involved in absorbing light for photosynthesis, fluoresces by absorbing blue light around 440 nm and emitting red light around 670 nm (it also absorbs 660 nm red light, which makes chlorophyll appear green-yellow).  This is different than phycocyanin (excitation around 620 nm, emission around 650 nm).  Thus these two compounds can be measured by their fluorescence.  If a fluorometer is tuned to detect light at 650 nm and to provide light at 620 nm then it will be able to detect phycocyanin.  On the other hand if its excitation and emission wavelengths are tuned to 440 nm and 670 nm, respectively, then it can detect chlorophyll.

A phycocyanin solution appears blue

A phycocyanin solution appears blue

This is useful for many types of applications because it eliminates the need for laborious separation of the compound.  Thus, chlorophyll and phycocyanin can be detected from a raw water sample without the need to extract, separate, and then characterize each sample.  For managers of lakes, ponds, and fountains, these two fluorescent compounds are important.  Changes in the amount of chlorophyll indicate changes in the amount of photosynthetic organisms.  This then is a reflection on conditions of the water.  Phycocyanin is mostly found in cyanobacteria and not other photosynthetic organisms.  Cyanobacteria are potentially hazardous because some strains have the ability to produce toxins that would then poison the water.  Therefore, phycocyanin fluorescence is measured to assess the amount of cyanobacteria and to guide further decisions for water management.

Product Description

004Three types of Amiscience fluorometers are available from Beagle Bioproducts.  The first measures chlorophyll (excitation 440 nm, emission 670 nm), the second measures phycocyanin (excitation 600 nm, emission 650 nm), and the third has two channels so that it can be used to measure both.  Each fluorometer is durable and portable so that it can be taken directly to a field site to perform on-the-spot measurements.  To make a measurement, a 0.2 mL polypropylene tube, similar to what some researchers use for PCR, is filled and placed in the chamber.  Then, through the user-friendly, touch-screen interface, measurements and calibrations are made quickly and easily.  Amiscience’s fluorometers can be calibrated by first measuring a sample that does not contain any fluorescent material, usually just water, then measuring a calibrator with a known concentration of material.  It can be difficult to find calibrators that are actually phycocyanin or chlorophyll, but Beagle is proud to offer a phycocyanin calibrator, soon to be followed by a chlorophyll calibrator.  The fluorometers only need to be recalibrated every few months if conditions are stable, such as for use in a laboratory-controlled environment.  The fluorometer should be recalibrated more frequently if conditions like temperature change.  The fluorometer can detect chlorophyll from 0.25 to 2500 ppb and phycocyanin from 10 – 100,000 ppb, making it versatile for not just water monitoring but for laboratory work as well.  Each fluorometer comes with a user’s manual that details multipoint calibrations if desired, as well as how to retain data in the instrument for later download.

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